Research in the field of molecular oncology concerns the development of new therapeutic strategies based on the regulation of gene expression with the use of non-coding RNA molecules (ncRNA). The aim of the research is to characterize the molecular phenotype of bone cancer (osteosarcoma) and liposarcoma (liposarcoma).
These studies are carried out on the basis of molecular biology techniques, including the use of high-pass RTq-qPCR tests (transcriptome analysis) or protein expression analysis (proteome analysis). Additionally, the molecular phenotype of neoplastic cells is assessed using flow cytometry and immunocytochemistry.
- Śmieszek, A., Marcinkowska, K., Pielok, A., Sikora, M., Valihrach, L., & Marycz, K. (2020). The Role of miR-21 in Osteoblasts-Osteoclasts Coupling In Vitro. Cells, 9(2), 1–21. http://doi.org/10.3390/cells9020479
- Sikora, M., Marcinkowska, K., Marycz, K., Wiglusz, R. J., & Śmieszek, A. (2019). The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells. Materials (Basel, Switzerland), 12(22), 3779. https://doi.org/10.3390/ma12223779
- Sikora, M., Marycz, K., & Smieszek, A. (2020). Small and Long Non-coding RNAs as Functional Regulators of Bone Homeostasis, Acting Alone or Cooperatively. Molecular therapy. Nucleic acids, 21, 792–803. https://doi.org/10.1016/j.omtn.2020.07.017